EXTRACTION OF FLAVOURING COMPOUND FROM ACTINOMYCETES

Abstract

The main objective of the present study was isolation, purification, and characterization of actinomycetes from soil samples, having a secondary metabolite production. Soils samples were taken from agricultural land. These samples were serially diluted and plated, on isolation of Actinomycetes agar media. Potential colonies were screened, purified, and stored in. Isolates were morphologically and biochemically characterized. These isolates were subjected to extraction for production of the Geosmin a chemical compound. Culture dependable isolation techniques were followed for the isolation of the active actinomycetes isolates and the secondary metabolite properties of the actinomcyetes were detected by modified Tryptic Soy Broth, and the distillation takes place. Then the crude extract of geosmin is separated using distillation method.

Key Words: - Geosmin, Isolation of Actinomycetes from soil, Chromatographic techniques, Extraction process.